Recombinant:
Article Title: Calcium transients on the ER surface trigger liquid-liquid phase separation of FIP200 to specify autophagosome initiation sites.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-FIP200 Proteintech Cat# 17250-1-AP; RRID:AB_1066642 8 Rabbit anti-FIP200 Sigma-Aldrich Cat# SAB4200135; RRID:AB_10621935 Mouse anti-ATG13 Merck Cat# MABC46; RRID:AB_11211663 Rabbit anti-ATG13 Cell Signaling Technology Cat# 13468S; RRID:AB_2797419 Mouse monoclonal anti-WIPI2 Abcam Cat# ab105459; RRID:AB_10860881 Rabbit anti-ATG9 MBL Cat# PD042; RRID:AB_2714019 Rabbit anti-EI24 Sigma Cat# HPA047165; RRID:AB_2679965 Rabbit anti-SERCA2 Cell Signaling Technology Cat# 4388S; RRID:AB_2227684 Mouse monoclonal anti-LC3 (clone 4E12) MBL Cat# M152-3; RRID: AB_1279144 Rabbit polyclonal anti-LC3 Cell Signaling Technology Cat# 2775S; RRID:AB_915 950 Rabbit polyclonal anti-p62 MBL Cat# PM045; RRID:AB_1279301 Rabbit anti-VAPA Proteintech Cat# 15275-1-AP; RRID:AB_2256991 Mouse anti-VAPB Proteintech Cat# 66191-1-IG; RRID:AB_2881586 Rabbit anti-S6K Cell Signaling Technology Cat# 2708S; RRID:AB_390722 Rabbit anti-p-S6K Cell Signaling Technology Cat# 9234L; RRID:AB_2269803 Mouse anti-Myc Sigma-Aldrich Cat# M5546; RRID:AB_260581 Mouse anti-His Sigma-Aldrich Cat# 70796-M; RRID:AB_10807496 Mouse monoclonal anti-GFP (clone 7.1 and 13.1) Roche Cat# 11814460001; RRID:AB_390913 Mouse monoclonal anti-Actin (clone 7D2C10) Proteintech Cat# 60008-1-Ig; RRID:AB_2289225 Mouse monoclonal anti-GAPDH Proteintech Cat# 60004-1-Ig; RRID:AB_2107436 Bacterial and virus strains E. coli BL21-CodonPlus (DE3) Agilent Cat# 280230 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium, high glucose HyClone Cat# SH30022.01B Fetal Bovine Serum ThermoFisher Scientific Cat# 10099-141C Hank’s balanced salt solution (HBSS) Gibco Cat# 14025-092 DMEM without amino acids HyClone Cat# SH4007.01 Thapsigargin (TG) Sigma Cat# T9033 BAPTA-AM Abcam Cat# AB120503 EGTA-AM ThermoFisher Scientific Cat# E1219 Isoprenaline (ISO) Thermo Scientific Cat# 11683009 Caffeine Thermo Scientific Cat# 11364569 Ryanodine Merck Cat# 559276 Anisomycin EMD Millipore Cat# 176880 Bafilomycin A1 Sigma Aldrich Cat# B1793 KB-R7943 Sigma Aldrich Cat# K4144 Torin 1 Cell Signaling Technology Cat# 14379 Chloroquine (CQ) Sigma Aldrich Cat# C6628 Poly-L-Lysine Sigma Aldrich Cat# P4832 Triton X-100 ThermoFisher Scientific Cat# HFH10 digitonin Sigma Cat# D141 protease inhibitor cocktail Roche Cat# 11836170001 (Continued on next page) Cell 185, 4082–4098.e1–e8, October 27, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene M&C Gene Technology Cat# MC032 Lipofectamine 2000 Life Technologies Cat# 12566014 Lipofectamine RNAi MAX Life Technologies Cat#13778150 proteinase K Sigma Cat# P2308 biotin Sigma Cat# B4639 1,6-hexanediol Sigma Cat# MKCP2300 membrane-impermeable ligand (MIL) HaloTag Alexa Fluor488 ligand Promega Cat# G1001 membrane-permeable ligand (MPL) HaloTag TMR ligand Promega Cat# G8251 Sepharose 4B beads GE Healthcare Cat# 17-0756-05 Ni-NTA agarose beads QIAGEN Cat# 30210 GFP-Trap agarose beads Lablead Cat# GNA-25-500 Mg-ATP Sigma-Aldrich Cat# A9187 EGTA Sigma-Aldrich Cat# E3889 phosphocreatine di(tris) Sigma-Aldrich Cat# P1937 creatine phosphokinase Sigma Cat# C37555 L-Glutathine reduced Sigma-Aldrich Cat# G4251 Fura-2-AM ThermoFisher Cat# F1221 Janelia Fluor 646 HaloTag ligand Promega Cat# GA1121 EMBED-812 Electron Microscopy Sciences Cat# 14120 glass-bottom dish NEST Cat# 801001 coverslip Matsunami Cat# C015001 Critical commercial assays ViraPower Lentiviral Expression Systems Invitrogen K497500 Experimental models: Cell lines HeLa cells ATCC CCL-2 COS7 cells ATCC N/A HEK293T cells ATCC CRL-11268 HEK293FT cells ATCC N/A EI24 KO U2OS cells from Dr. Pingyong Xu’s lab (Institute of Biophysics, Chinese Academy of Sciences) N/A EI24 KO MEF cells This paper N/A EI24 KO N2a cells This paper N/A Oligonucleotides Primers used for qRT-PCR experiments, see Table S1 This paper N/A siRNA and gRNA sequences, see Table S1 This paper N/A Recombinant DNA GFP-FIP200 Laboratory of Dr. Noboru Mizushima (University of Tokyo) N/A HaloTag-FIP200 This paper N/A ULK1-GFP Zhao et al., 2017 N/A mCherry-FIP200 Zhao et al., 2017 N/A GFP-ATG13 Miao et al., 2020 N/A GFP-ATG101 Liu et al., 2021 N/A GFP/mCherry-ATG14 Zhao et al., 2017 N/A (Continued on next page) ll e2 Cell 185, 4082–4098.e1–e8, October 27, 2022 Article
Modification:
Article Title: Calcium transients on the ER surface trigger liquid-liquid phase separation of FIP200 to specify autophagosome initiation sites.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-FIP200 Proteintech Cat# 17250-1-AP; RRID:AB_1066642 8 Rabbit anti-FIP200 Sigma-Aldrich Cat# SAB4200135; RRID:AB_10621935 Mouse anti-ATG13 Merck Cat# MABC46; RRID:AB_11211663 Rabbit anti-ATG13 Cell Signaling Technology Cat# 13468S; RRID:AB_2797419 Mouse monoclonal anti-WIPI2 Abcam Cat# ab105459; RRID:AB_10860881 Rabbit anti-ATG9 MBL Cat# PD042; RRID:AB_2714019 Rabbit anti-EI24 Sigma Cat# HPA047165; RRID:AB_2679965 Rabbit anti-SERCA2 Cell Signaling Technology Cat# 4388S; RRID:AB_2227684 Mouse monoclonal anti-LC3 (clone 4E12) MBL Cat# M152-3; RRID: AB_1279144 Rabbit polyclonal anti-LC3 Cell Signaling Technology Cat# 2775S; RRID:AB_915 950 Rabbit polyclonal anti-p62 MBL Cat# PM045; RRID:AB_1279301 Rabbit anti-VAPA Proteintech Cat# 15275-1-AP; RRID:AB_2256991 Mouse anti-VAPB Proteintech Cat# 66191-1-IG; RRID:AB_2881586 Rabbit anti-S6K Cell Signaling Technology Cat# 2708S; RRID:AB_390722 Rabbit anti-p-S6K Cell Signaling Technology Cat# 9234L; RRID:AB_2269803 Mouse anti-Myc Sigma-Aldrich Cat# M5546; RRID:AB_260581 Mouse anti-His Sigma-Aldrich Cat# 70796-M; RRID:AB_10807496 Mouse monoclonal anti-GFP (clone 7.1 and 13.1) Roche Cat# 11814460001; RRID:AB_390913 Mouse monoclonal anti-Actin (clone 7D2C10) Proteintech Cat# 60008-1-Ig; RRID:AB_2289225 Mouse monoclonal anti-GAPDH Proteintech Cat# 60004-1-Ig; RRID:AB_2107436 Bacterial and virus strains E. coli BL21-CodonPlus (DE3) Agilent Cat# 280230 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium, high glucose HyClone Cat# SH30022.01B Fetal Bovine Serum ThermoFisher Scientific Cat# 10099-141C Hank’s balanced salt solution (HBSS) Gibco Cat# 14025-092 DMEM without amino acids HyClone Cat# SH4007.01 Thapsigargin (TG) Sigma Cat# T9033 BAPTA-AM Abcam Cat# AB120503 EGTA-AM ThermoFisher Scientific Cat# E1219 Isoprenaline (ISO) Thermo Scientific Cat# 11683009 Caffeine Thermo Scientific Cat# 11364569 Ryanodine Merck Cat# 559276 Anisomycin EMD Millipore Cat# 176880 Bafilomycin A1 Sigma Aldrich Cat# B1793 KB-R7943 Sigma Aldrich Cat# K4144 Torin 1 Cell Signaling Technology Cat# 14379 Chloroquine (CQ) Sigma Aldrich Cat# C6628 Poly-L-Lysine Sigma Aldrich Cat# P4832 Triton X-100 ThermoFisher Scientific Cat# HFH10 digitonin Sigma Cat# D141 protease inhibitor cocktail Roche Cat# 11836170001 (Continued on next page) Cell 185, 4082–4098.e1–e8, October 27, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene M&C Gene Technology Cat# MC032 Lipofectamine 2000 Life Technologies Cat# 12566014 Lipofectamine RNAi MAX Life Technologies Cat#13778150 proteinase K Sigma Cat# P2308 biotin Sigma Cat# B4639 1,6-hexanediol Sigma Cat# MKCP2300 membrane-impermeable ligand (MIL) HaloTag Alexa Fluor488 ligand Promega Cat# G1001 membrane-permeable ligand (MPL) HaloTag TMR ligand Promega Cat# G8251 Sepharose 4B beads GE Healthcare Cat# 17-0756-05 Ni-NTA agarose beads QIAGEN Cat# 30210 GFP-Trap agarose beads Lablead Cat# GNA-25-500 Mg-ATP Sigma-Aldrich Cat# A9187 EGTA Sigma-Aldrich Cat# E3889 phosphocreatine di(tris) Sigma-Aldrich Cat# P1937 creatine phosphokinase Sigma Cat# C37555 L-Glutathine reduced Sigma-Aldrich Cat# G4251 Fura-2-AM ThermoFisher Cat# F1221 Janelia Fluor 646 HaloTag ligand Promega Cat# GA1121 EMBED-812 Electron Microscopy Sciences Cat# 14120 glass-bottom dish NEST Cat# 801001 coverslip Matsunami Cat# C015001 Critical commercial assays ViraPower Lentiviral Expression Systems Invitrogen K497500 Experimental models: Cell lines HeLa cells ATCC CCL-2 COS7 cells ATCC N/A HEK293T cells ATCC CRL-11268 HEK293FT cells ATCC N/A EI24 KO U2OS cells from Dr. Pingyong Xu’s lab (Institute of Biophysics, Chinese Academy of Sciences) N/A EI24 KO MEF cells This paper N/A EI24 KO N2a cells This paper N/A Oligonucleotides Primers used for qRT-PCR experiments, see Table S1 This paper N/A siRNA and gRNA sequences, see Table S1 This paper N/A Recombinant DNA GFP-FIP200 Laboratory of Dr. Noboru Mizushima (University of Tokyo) N/A HaloTag-FIP200 This paper N/A ULK1-GFP Zhao et al., 2017 N/A mCherry-FIP200 Zhao et al., 2017 N/A GFP-ATG13 Miao et al., 2020 N/A GFP-ATG101 Liu et al., 2021 N/A GFP/mCherry-ATG14 Zhao et al., 2017 N/A (Continued on next page) ll e2 Cell 185, 4082–4098.e1–e8, October 27, 2022 Article
Protease Inhibitor:
Article Title: Calcium transients on the ER surface trigger liquid-liquid phase separation of FIP200 to specify autophagosome initiation sites.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-FIP200 Proteintech Cat# 17250-1-AP; RRID:AB_1066642 8 Rabbit anti-FIP200 Sigma-Aldrich Cat# SAB4200135; RRID:AB_10621935 Mouse anti-ATG13 Merck Cat# MABC46; RRID:AB_11211663 Rabbit anti-ATG13 Cell Signaling Technology Cat# 13468S; RRID:AB_2797419 Mouse monoclonal anti-WIPI2 Abcam Cat# ab105459; RRID:AB_10860881 Rabbit anti-ATG9 MBL Cat# PD042; RRID:AB_2714019 Rabbit anti-EI24 Sigma Cat# HPA047165; RRID:AB_2679965 Rabbit anti-SERCA2 Cell Signaling Technology Cat# 4388S; RRID:AB_2227684 Mouse monoclonal anti-LC3 (clone 4E12) MBL Cat# M152-3; RRID: AB_1279144 Rabbit polyclonal anti-LC3 Cell Signaling Technology Cat# 2775S; RRID:AB_915 950 Rabbit polyclonal anti-p62 MBL Cat# PM045; RRID:AB_1279301 Rabbit anti-VAPA Proteintech Cat# 15275-1-AP; RRID:AB_2256991 Mouse anti-VAPB Proteintech Cat# 66191-1-IG; RRID:AB_2881586 Rabbit anti-S6K Cell Signaling Technology Cat# 2708S; RRID:AB_390722 Rabbit anti-p-S6K Cell Signaling Technology Cat# 9234L; RRID:AB_2269803 Mouse anti-Myc Sigma-Aldrich Cat# M5546; RRID:AB_260581 Mouse anti-His Sigma-Aldrich Cat# 70796-M; RRID:AB_10807496 Mouse monoclonal anti-GFP (clone 7.1 and 13.1) Roche Cat# 11814460001; RRID:AB_390913 Mouse monoclonal anti-Actin (clone 7D2C10) Proteintech Cat# 60008-1-Ig; RRID:AB_2289225 Mouse monoclonal anti-GAPDH Proteintech Cat# 60004-1-Ig; RRID:AB_2107436 Bacterial and virus strains E. coli BL21-CodonPlus (DE3) Agilent Cat# 280230 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium, high glucose HyClone Cat# SH30022.01B Fetal Bovine Serum ThermoFisher Scientific Cat# 10099-141C Hank’s balanced salt solution (HBSS) Gibco Cat# 14025-092 DMEM without amino acids HyClone Cat# SH4007.01 Thapsigargin (TG) Sigma Cat# T9033 BAPTA-AM Abcam Cat# AB120503 EGTA-AM ThermoFisher Scientific Cat# E1219 Isoprenaline (ISO) Thermo Scientific Cat# 11683009 Caffeine Thermo Scientific Cat# 11364569 Ryanodine Merck Cat# 559276 Anisomycin EMD Millipore Cat# 176880 Bafilomycin A1 Sigma Aldrich Cat# B1793 KB-R7943 Sigma Aldrich Cat# K4144 Torin 1 Cell Signaling Technology Cat# 14379 Chloroquine (CQ) Sigma Aldrich Cat# C6628 Poly-L-Lysine Sigma Aldrich Cat# P4832 Triton X-100 ThermoFisher Scientific Cat# HFH10 digitonin Sigma Cat# D141 protease inhibitor cocktail Roche Cat# 11836170001 (Continued on next page) Cell 185, 4082–4098.e1–e8, October 27, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene M&C Gene Technology Cat# MC032 Lipofectamine 2000 Life Technologies Cat# 12566014 Lipofectamine RNAi MAX Life Technologies Cat#13778150 proteinase K Sigma Cat# P2308 biotin Sigma Cat# B4639 1,6-hexanediol Sigma Cat# MKCP2300 membrane-impermeable ligand (MIL) HaloTag Alexa Fluor488 ligand Promega Cat# G1001 membrane-permeable ligand (MPL) HaloTag TMR ligand Promega Cat# G8251 Sepharose 4B beads GE Healthcare Cat# 17-0756-05 Ni-NTA agarose beads QIAGEN Cat# 30210 GFP-Trap agarose beads Lablead Cat# GNA-25-500 Mg-ATP Sigma-Aldrich Cat# A9187 EGTA Sigma-Aldrich Cat# E3889 phosphocreatine di(tris) Sigma-Aldrich Cat# P1937 creatine phosphokinase Sigma Cat# C37555 L-Glutathine reduced Sigma-Aldrich Cat# G4251 Fura-2-AM ThermoFisher Cat# F1221 Janelia Fluor 646 HaloTag ligand Promega Cat# GA1121 EMBED-812 Electron Microscopy Sciences Cat# 14120 glass-bottom dish NEST Cat# 801001 coverslip Matsunami Cat# C015001 Critical commercial assays ViraPower Lentiviral Expression Systems Invitrogen K497500 Experimental models: Cell lines HeLa cells ATCC CCL-2 COS7 cells ATCC N/A HEK293T cells ATCC CRL-11268 HEK293FT cells ATCC N/A EI24 KO U2OS cells from Dr. Pingyong Xu’s lab (Institute of Biophysics, Chinese Academy of Sciences) N/A EI24 KO MEF cells This paper N/A EI24 KO N2a cells This paper N/A Oligonucleotides Primers used for qRT-PCR experiments, see Table S1 This paper N/A siRNA and gRNA sequences, see Table S1 This paper N/A Recombinant DNA GFP-FIP200 Laboratory of Dr. Noboru Mizushima (University of Tokyo) N/A HaloTag-FIP200 This paper N/A ULK1-GFP Zhao et al., 2017 N/A mCherry-FIP200 Zhao et al., 2017 N/A GFP-ATG13 Miao et al., 2020 N/A GFP-ATG101 Liu et al., 2021 N/A GFP/mCherry-ATG14 Zhao et al., 2017 N/A (Continued on next page) ll e2 Cell 185, 4082–4098.e1–e8, October 27, 2022 Article
Electron Microscopy:
Virus:
Article Title: Calcium transients on the ER surface trigger liquid-liquid phase separation of FIP200 to specify autophagosome initiation sites.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-FIP200 Proteintech Cat# 17250-1-AP; RRID:AB_1066642 8 Rabbit anti-FIP200 Sigma-Aldrich Cat# SAB4200135; RRID:AB_10621935 Mouse anti-ATG13 Merck Cat# MABC46; RRID:AB_11211663 Rabbit anti-ATG13 Cell Signaling Technology Cat# 13468S; RRID:AB_2797419 Mouse monoclonal anti-WIPI2 Abcam Cat# ab105459; RRID:AB_10860881 Rabbit anti-ATG9 MBL Cat# PD042; RRID:AB_2714019 Rabbit anti-EI24 Sigma Cat# HPA047165; RRID:AB_2679965 Rabbit anti-SERCA2 Cell Signaling Technology Cat# 4388S; RRID:AB_2227684 Mouse monoclonal anti-LC3 (clone 4E12) MBL Cat# M152-3; RRID: AB_1279144 Rabbit polyclonal anti-LC3 Cell Signaling Technology Cat# 2775S; RRID:AB_915 950 Rabbit polyclonal anti-p62 MBL Cat# PM045; RRID:AB_1279301 Rabbit anti-VAPA Proteintech Cat# 15275-1-AP; RRID:AB_2256991 Mouse anti-VAPB Proteintech Cat# 66191-1-IG; RRID:AB_2881586 Rabbit anti-S6K Cell Signaling Technology Cat# 2708S; RRID:AB_390722 Rabbit anti-p-S6K Cell Signaling Technology Cat# 9234L; RRID:AB_2269803 Mouse anti-Myc Sigma-Aldrich Cat# M5546; RRID:AB_260581 Mouse anti-His Sigma-Aldrich Cat# 70796-M; RRID:AB_10807496 Mouse monoclonal anti-GFP (clone 7.1 and 13.1) Roche Cat# 11814460001; RRID:AB_390913 Mouse monoclonal anti-Actin (clone 7D2C10) Proteintech Cat# 60008-1-Ig; RRID:AB_2289225 Mouse monoclonal anti-GAPDH Proteintech Cat# 60004-1-Ig; RRID:AB_2107436 Bacterial and virus strains E. coli BL21-CodonPlus (DE3) Agilent Cat# 280230 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium, high glucose HyClone Cat# SH30022.01B Fetal Bovine Serum ThermoFisher Scientific Cat# 10099-141C Hank’s balanced salt solution (HBSS) Gibco Cat# 14025-092 DMEM without amino acids HyClone Cat# SH4007.01 Thapsigargin (TG) Sigma Cat# T9033 BAPTA-AM Abcam Cat# AB120503 EGTA-AM ThermoFisher Scientific Cat# E1219 Isoprenaline (ISO) Thermo Scientific Cat# 11683009 Caffeine Thermo Scientific Cat# 11364569 Ryanodine Merck Cat# 559276 Anisomycin EMD Millipore Cat# 176880 Bafilomycin A1 Sigma Aldrich Cat# B1793 KB-R7943 Sigma Aldrich Cat# K4144 Torin 1 Cell Signaling Technology Cat# 14379 Chloroquine (CQ) Sigma Aldrich Cat# C6628 Poly-L-Lysine Sigma Aldrich Cat# P4832 Triton X-100 ThermoFisher Scientific Cat# HFH10 digitonin Sigma Cat# D141 protease inhibitor cocktail Roche Cat# 11836170001 (Continued on next page) Cell 185, 4082–4098.e1–e8, October 27, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene M&C Gene Technology Cat# MC032 Lipofectamine 2000 Life Technologies Cat# 12566014 Lipofectamine RNAi MAX Life Technologies Cat#13778150 proteinase K Sigma Cat# P2308 biotin Sigma Cat# B4639 1,6-hexanediol Sigma Cat# MKCP2300 membrane-impermeable ligand (MIL) HaloTag Alexa Fluor488 ligand Promega Cat# G1001 membrane-permeable ligand (MPL) HaloTag TMR ligand Promega Cat# G8251 Sepharose 4B beads GE Healthcare Cat# 17-0756-05 Ni-NTA agarose beads QIAGEN Cat# 30210 GFP-Trap agarose beads Lablead Cat# GNA-25-500 Mg-ATP Sigma-Aldrich Cat# A9187 EGTA Sigma-Aldrich Cat# E3889 phosphocreatine di(tris) Sigma-Aldrich Cat# P1937 creatine phosphokinase Sigma Cat# C37555 L-Glutathine reduced Sigma-Aldrich Cat# G4251 Fura-2-AM ThermoFisher Cat# F1221 Janelia Fluor 646 HaloTag ligand Promega Cat# GA1121 EMBED-812 Electron Microscopy Sciences Cat# 14120 glass-bottom dish NEST Cat# 801001 coverslip Matsunami Cat# C015001 Critical commercial assays ViraPower Lentiviral Expression Systems Invitrogen K497500 Experimental models: Cell lines HeLa cells ATCC CCL-2 COS7 cells ATCC N/A HEK293T cells ATCC CRL-11268 HEK293FT cells ATCC N/A EI24 KO U2OS cells from Dr. Pingyong Xu’s lab (Institute of Biophysics, Chinese Academy of Sciences) N/A EI24 KO MEF cells This paper N/A EI24 KO N2a cells This paper N/A Oligonucleotides Primers used for qRT-PCR experiments, see Table S1 This paper N/A siRNA and gRNA sequences, see Table S1 This paper N/A Recombinant DNA GFP-FIP200 Laboratory of Dr. Noboru Mizushima (University of Tokyo) N/A HaloTag-FIP200 This paper N/A ULK1-GFP Zhao et al., 2017 N/A mCherry-FIP200 Zhao et al., 2017 N/A GFP-ATG13 Miao et al., 2020 N/A GFP-ATG101 Liu et al., 2021 N/A GFP/mCherry-ATG14 Zhao et al., 2017 N/A (Continued on next page) ll e2 Cell 185, 4082–4098.e1–e8, October 27, 2022 Article
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